Chemistry Forum

Hi Guys, I have a few doubts about Michaelis-Menten Enzymes.

1)Does the reaction rate in a reaction using michaelis-menten enzymes depend only on the concentration of the substrate?
2)fter the discussion of Mentenian kinetics, the books report competitive, non-competitive and incompetitive enzyme inhibitors, indicating their effects on Km and Vmax, which are typical of Mentenian kinetics. But can't these inhibitors also act on non-Mentenian enzymes?
Thanks in advance

1. Mainly yes, but also temperature, flow rate etc. has influence.
2. Probably yes, depends on the Enzym.

ChenBeier wrote: ↑Mon Mar 03, 2025 4:38 am 1. Mainly yes, but also temperature, flow rate etc. has influence.
2. Probably yes, depends on the Enzym.

Thanks <3

Speed Stars Online Besides substrate concentration, what other factors influence the reaction rate in Michaelis-Menten kinetics such as enzyme concentration, temperature, or pH?

All of them.

sottozer0 wrote: ↑Sun Mar 02, 2025 3:52 pm Hi Guys, I have a few doubts about Michaelis-Menten Enzymes.

1)Does the reaction rate in a reaction using michaelis-menten enzymes depend only on the concentration of the substrate?
2)fter the discussion of Mentenian kinetics, the books report competitive, non-competitive and incompetitive enzyme inhibitors, indicating their effects on Km and Vmax, which are typical of Mentenian kinetics. But can't these inhibitors also act on non-Mentenian enzymes?
Thanks in advance

Great questions! 1) The reaction rate depends on both substrate concentration and enzyme characteristics like Km and Vmax, not just substrate. 2) Yes, those inhibitors can also affect non-Michaelis-Menten enzymes, but their effects may not follow the classic patterns seen in Mentenian kinetics. Hope that helps!

bevis0405 wrote: ↑Tue Jun 17, 2025 2:01 am Melon Playground Besides substrate concentration, what other factors influence the reaction rate in Michaelis-Menten kinetics such as enzyme concentration, temperature, or pH?

The reaction rate of MM enzymes depends on more than just substrate concentration—it also depends on enzyme concentration,Vₘₐₓ, Kₘ, and environmental conditions. Inhibitors like competitive and non-competitive types can affect non-MM enzymes, but their effects may not align with MM expectations. More advanced models may be needed in those cases.

When studying Michaelis-Menten kinetics, many factors influence the reaction rate in addition to substrate concentration. Enzyme concentration is an important factor; increasing enzyme concentration generally increases the reaction rate until saturation. Temperature and pH also have significant effects, with each enzyme having its own optimum. In addition, inhibitors such as competitive and non-competitive types can alter the reaction rate, especially for enzymes that do not follow the Michaelis-Menten law. These factors must be adjusted to optimize the reaction.