Amino acids and metabolites
Posted: Fri Mar 01, 2024 6:54 am
Hi! Im going to do a little research for my internship about the detection of amino acids and some metabolites in blood with the HPLC/UPLC. And with this reseacrh I will try to determine if Its possible to determine the date of deposition of the bloodtrace.
I have to chose which amino acids and metabolites I want to reseach, and I have some trouble with picking. I would prefer amino acids and metabolites which are fluorescent, so I don't have to do a derivatization step. I was thinking about Trypophan, Phenylalanine and Tyrosine. But I am not sure if these amino acids are ''too big'' to detect with the HPLC? I also don't really know if I should pick metabolites which are formed from these amino acids, like kyruenine and norhaman. or to pick just random other metabolites. My teacher talked about picking oxidation metabolites but I can't really find what the oxidation metabolites are of Trypophan, Phenylalanine and Tyrosine.
Help is needed! Thankyou in advance.
I have to chose which amino acids and metabolites I want to reseach, and I have some trouble with picking. I would prefer amino acids and metabolites which are fluorescent, so I don't have to do a derivatization step. I was thinking about Trypophan, Phenylalanine and Tyrosine. But I am not sure if these amino acids are ''too big'' to detect with the HPLC? I also don't really know if I should pick metabolites which are formed from these amino acids, like kyruenine and norhaman. or to pick just random other metabolites. My teacher talked about picking oxidation metabolites but I can't really find what the oxidation metabolites are of Trypophan, Phenylalanine and Tyrosine.
Help is needed! Thankyou in advance.