Chemistry Forum

Hi! Im going to do a little research for my internship about the detection of amino acids and some metabolites in blood with the HPLC/UPLC. And with this reseacrh I will try to determine if Its possible to determine the date of deposition of the bloodtrace.

I have to chose which amino acids and metabolites I want to reseach, and I have some trouble with picking. I would prefer amino acids and metabolites which are fluorescent, so I don't have to do a derivatization step. I was thinking about Trypophan, Phenylalanine and Tyrosine. But I am not sure if these amino acids are ''too big'' to detect with the HPLC? I also don't really know if I should pick metabolites which are formed from these amino acids, like kyruenine and norhaman. or to pick just random other metabolites. My teacher talked about picking oxidation metabolites but I can't really find what the oxidation metabolites are of Trypophan, Phenylalanine and Tyrosine.

Help is needed! Thankyou in advance.

To avoid the derivatization process, I would prefer to use fluorescent amino acids and metabolites.
amanda the adventurer

For your internship research on detecting amino acids and metabolites in blood using HPLC/UPLC, focusing on fluorescent amino acids like tryptophan, phenylalanine, and tyrosine is a solid choice. These amino acids are commonly studied using HPLC/UPLC and don't require derivatization. Considering metabolites related to these amino acids, such as kynurenine for tryptophan, can provide valuable insights into their metabolic pathways. Additionally, exploring oxidation metabolites like 3-hydroxykynurenine and quinolinic acid derived from these amino acids' oxidation can deepen your understanding.